BABET-REAL5 research results from Argentina published

Biomass bioconversion results, obtained by Dr. Eleonora Campos’s research group from Argentina, were recently published in Bioenergy Research journal, Springer. The work focused on the development of xylanases to complement commercial enzymatic cocktails, which is part of the key innovation issues concerning to BABET-REAL 5 project.

The research team from Argentina (from left): Dr. Ornella Ontrañon, Dr. Silvina Ghio, Dr. Eleonora Campos and MSc. Florencia Piccinni

Multiple potentially secreted enzymes involved in polysaccharide deconstruction are encoded in the genome of Paenibacillus sp. A59, a xylanolytic soil bacterium, including glycoside hydrolases which were identified in secretome analysis by culture on xylan or biomass. The original article included the thorough characterization of two recombinant endoxylanases from Paenibacillus sp. A59, GH10XynA and GH11XynB, and their application in biomass hydrolysis. Pre- treated wheat straw, barley straw and sweet corn cob were obtained by extrusion at CIEMAT-Spain and INPT, and were subjected to hydrolysis by the novel enzymes taking efficient commercial enzymatic cocktails as benchmark. Both endoxylanases released mainly xylobiose and xylotriose from biomasses, and GH10XynA also released xylose. Results published in the article highlighted the potential application of both endoxylanases in industrial bioprocesses on lignocellulosic biomass.

The paper was electronically published and can be accessed with the following link:

http://link.springer.com/article/10.1007/s12155-017-9887-7